Sentence examples for lymphocytes were seeded from inspiring English sources
Lymphocytes were seeded in round bottom 96 well plates with syngeneic irradiated BMDCs pulsed with the stimuli described above and co-cultured for 4 days.
A total of 2 × 10 lymphocytes were seeded per well and incubated with 5 μg/ml of RAH or control peptide (VVYRDSIPH) in 200 μl of RPMI for 48 h.
B lymphocytes were seeded at 2 to 3 × 10 cells/mL in 6-well Primaria plates (BD Biosciences, Mississauga, Canada) in the presence of 4 to 6 × 10 cells/mL γ-irradiated CD154+ L4.5 cells [ 11].
For this study, 10 U-87 MG cells were first seeded on a TCP as the lower chamber of the transwell device and T lymphocytes were seeded onto transwell plates precoated with 200 μL of the PCgel or Matrigel for 1 day.
To investigate if T lymphocytes invading through gels specifically bind to tumor cells, 10 U-87 MG cells were seeded on a TCP as the lower chamber of the transwell device and T lymphocytes were seeded onto transwell plates precoated with the PCgel or Matrigel (as control) the following day.
Purified T-lymphocytes were seeded on Chamberslides (Lab-Tek) at 1×105 cells/well and were stimulated or not with anti-CD3 and anti-CD28 polyclonal antibodies for 24 h.
B lymphocyte Ramos-Blue cells were seeded at a density of 2 × 106 cells/mL, and the cells were immediately stimulated with 0.5 μM ODNs.
Lymphocytes (1 × 10/well) isolated from SLNs and PBMCs were seeded in 96-well plates that were pre-coated with an anti-human IFN-γ antibody in triplicate and incubated with autologous tumor lysates or an anti-CD3 mAb.
Normal human lymphocytes and oral squamous epithelial cell, Jurkat and KB cell lines were seeded into 96 wells of tissue culture plates having 180 μl of complete media and were incubated for 48 h.
After three times of washing, serial dilutions of coated beads were seeded onto 96-well microtiter plates (15±10 0.6±10 beads/well) and activated lymphocytes (2 × 10 cells well) were added.
Bronchial epithelial cells were seeded over this gel and then cultured in an air-liquid interface in the presence or the absence of T lymphocytes.
