Sentence examples for lymphocyte suspension from inspiring English sources

For the adoptive transfer, 100 µL of the lymphocyte suspension (5×107 cells) or 300 µL of fresh serum or combination of lymphocyte suspension and serum from positive donors or negative donors was injected intravenously (i.v).v

Purified B lymphocyte suspension contained more than 95% B cells, and were cryopreserved in liquid nitrogen and thawed immediately before use.

For isolation of T regulatory cells, peripheral lymphocyte suspension from Foxn1+/Δ mice was stained by anti-CD4 PE and anti-CD25 allophycocyanin mAbs, then sorted by MoFlo FACS (DakoCytomation).

RNA was manually extracted from the lymphocyte suspension.

Irradiated EBV-BCL (50 Gy; Cs source) were added to the T lymphocyte suspension at a ratio of 2 : 1 (stimulators : responders).

Cells were subsequently resuspended in PBS (~ 100 μl/50 mg wet weight) with protease inhibitors to yield a lymphocyte suspension with protein concentration ~ 10 mg/ml.

A modification of the Boyden chamber system was employed to quantify lymphocyte motility by placing lymphocyte suspensions on 8-mum convoluted-pore nitrocellulose filters and measuring the depth of migration of the cells into the filter at 37 degrees C.

Thymocyte and lymphocyte suspensions were prepared and were stained for the expression of cell surface markers as described [48].

Peripheral lymphocyte suspensions were prepared from freshly isolated spleen and lymph nodes from C57BL6/J Ly5.1 or OT1 donor mice and further isolated by density gradient centrifugation and lysis of RBC in ACK lysis buffer (Cambrex Bio Science, Walkersville, MD).

A humoral response was indeed documented with anti-HLA I –but not II– antibodies in the blood (6 out of 30, i.e. cytotoxicity equivalent to 20% for a panel of 30 total lymphocyte suspensions) and in the cerebrospinal fluid (2 out of 30, i.e. cytotoxicity equivalent to 7% of the panel tested).

Lymphocyte suspensions were prepared from spleens and lymph nodes.