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(BFAs) has been proposed as a way to direct immune cells to the tumor: one arm of the antibody is specific for a known tumor-associated antigen and the other for a lymphocyte marker such as CD3.
The T lymphocyte marker CD45RA was used to determine the memory population (CD45RA−), and the naïve plus effector populations (CD45RA+).
Vγ1.1+Vδ6.3+ T cells share multiple characteristics with natural killer (NK) T cells including expression of the activation markers CD44, and NK1.1, and low expression of the immature T lymphocyte marker CD24.
To determine the extent to which the tissues examined were infiltrated by blood cells, we analyzed expression of CD79a mRNA (a B lymphocyte marker) in each of the tissues.
CD3 is a T lymphocyte marker.
Flow cytometric analysis (FCA) for markers CD45 (leukocyte common antigen), CD14 (monocyte/macrophage marker), CD3 (T lymphocyte marker), CD19 (B lymphocyte marker), kappa (kappa light chain B cell marker), lambda (lambda light chain B lymphocyte marker), CD4 (T helper lymphocyte marker), and CD8 (T cytotoxic lymphocyte marker) was done from vitreous and peripheral venous blood samples.
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In particular, the presence of lymphocyte markers including CD20, FoxP3, and T cell intracellular antigen-1 (TIA-1) are indicators of positive prognosis for patients manifesting HGSC (Milne et al., 2009), indicating recruitment of distinct populations of T cells to the tumor site to generate cytotoxic effects.
For most samples the expression levels of lymphocyte markers were equal.
Approximately 90% of the SWAT and MWAT samples included high levels of lymphocyte markers, and to obtain within-depot uniformity, SWAT and MWAT samples with no expression of lymphocyte markers were excluded.
Mesenteric and subcutaneous adipose tissue exhibited expression of lymphocyte markers reflecting the abundance of lymph nodes within these depots [14].
In contrast to lymphocyte markers, the dendritic cell marker CD1a showed no association with DSS, possibly due to the low number of tumors containing CD1a+ cells.
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