Exact(10)
The upper layer was drawn off using a clean Pasteur pipette, leaving the lymphocyte layer undisturbed at the interface.
The lymphocyte layer was carefully collected and transferred to a clean centrifuge tube using a clean Pasteur pipette.
The upper plasma layer was drawn off, leaving the lymphocyte layer undisturbed at the interface.
The lymphocyte layer was washed once with phosphate-buffered saline and recentrifuged.
The upper layer was removed using a clean Pasteur pipette and the lymphocyte layer was carefully collected.
The formed lymphocyte layer was transferred by pipette to another tube, and washed with PBS by centrifugation at 100 g for 10 min at 18 20°C.
Similar(50)
This generally consisted of a variable number of lymphocyte layers which were arranged around blood vessels and encased by a thin capsule of fibrous connective tissue.
Using a clean Pasteur pipette the lymphocytes layer will be harvested from the interface and transferred to a clean centrifuge tube.
After centrifuging at room temperature (400 g for 30 40 min), drawing of the upper layer by a clean Pasteur pipette will be done leaving the lymphocytes layer undisturbed at the interface.
The macrophages were isolated from the buffy coat layer (lymphocytes, macrophages) by adherence onto a glass dish.
Blood was layered on a two-layer density gradient consisting of lymphocyte separation medium 1077 (upper layer, PAA, Pasching, Austria) and Histopaque® 1119 (bottom layer, Sigma, Deisenhofen, Germany) and centrifuged for 5 min at 300 × g followed by 20 min at 800 × g.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com