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According to Allbritton, the system enables isolation strategies that might otherwise be impossible, such as isolating cytotoxic T lymphocytes based on their ability to kill target cells.
CD4+ lymphocytes analysis was done using three-color flow cytometry with antibodies against human-CD95, a prototype cell death receptor, and the chemokine receptor-7 (CCR7) after gating for lymphocytes based on the forward and side scatter.
Cells were initially defined as lymphocytes based on forward- and side-scatter profiles.
Thus, NK cells were herein identified as CD161bright lymphocytes, based on FITC-conjugated anti-CD161 labeling and scatter properties [48].
A gate was set to the region corresponding to the lymphocytes, based on the forward and side scatter diagram.
The purity of the CD4+ T lymphocytes based on the cell surface expression of CD3 and CD4 proteins was confirmed by flow cytometry to be ≥96%.
Positive gating for lymphocytes based on forward and side scatter was followed by CD3+CD4+ and CD3+CD8+ gating, and specific populations were further defined by using antibodies specific for CD8 and CD4, respectively.
Compelling circumstantial and experimental evidence suggests that the primary immunopathogenesis of psoriasis is T-lymphocyte based, so least in part it resembles sarcoidosis.
Analysis was conducted on ≥10 000 gated viable lymphocytes based on fluorescence minus one controls.
Positive gating for lymphocytes based on forward and side scatter was followed by CD3+ CD4+ and CD3+ CD8+ gating.
First, unsupervised HC clearly distinguished PTCLs/NOS from naive and activated T-lymphocytes based on global miRNA expression patterns.
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