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Optimized lung preparation for detailed structural evaluation is required to improve consistency in preclinical safety evaluation, differences of opinion exist among regulatory agency personnel regarding the optimal methods for routine formalin fixation of lungs from rodent toxicology studies.
The isolated lung preparation has been described in detail previously [18].
Lung preparation was performed as described previously [ 5].
For whole mount lung preparation, each mouse was sacrificed by anesthetic overdose.
An isolated perfused rat lung preparation (IPL) is described and its physiologic status is evaluated.
The isolated lung preparation remained in the thoracic cavity lying supine and was warmed with a heating lamp.
Similar(45)
All lung preparations received papaverine 0.1 μg/kg added to the perfusate for vasoplegia.
Styrene oxide biotransformation was studied in isolated, perfused rat liver and rabbit lung preparations.
For comparison, additional excised lung preparations were exposed to solutions of hydrogen peroxide either externally or intratracheally.
Thus, lung preparations from twenty-four rabbitspecificallylly, seven animals per HiP group and five animals per LoP group) were used.
To test these hypotheses, excised whole rat lung preparations were exposed to either internal (intratracheal) or external (pleural surface) smoke.
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