These parameters include: retention time in the lung, the amount and type of "fluid" available for dissolution, physiology of the various regions of the lung, lung dose, and deposition patterns.
The other three systems [11, 13, 18] (Table 2) give separate scores for both lungs, central versus peripheral lung, lung zones, quadrants or lobes to each of the different pulmonary abnormalities such as increased lung volume, bronchial wall thickening, mucus plugging, bronchiectasis and large lesions.
The following topical areas were explored as refinements to address the differences/similarities between the GI and the lung: Lung permeability and transport The role of fluid mechanics in pulmonary drug deposition Particle dissolution in the lung and in vitro testing as applicable to lung particle dissolution PK/PD modeling for lung delivery.
For isolation of lymphocytes in the lung, lung tissues were ground with fine metal strainer, followed by digestion with HBSS containing 5% FBS, 125 U/mL collagenase I (GIBCO Inc., Grand Island, NY, USA), 60 U/mL DNase I and 60 U/mL hyaluronidase (Sigma, St Louis, MO, USA) for 60 min at 37°C.
To obtain different lung conditions to test our hypothesis we used three models: normal lung, lung collapse, and mechanical lung injury.
The Steel Dwass method was used to compare CCL3 levels among the asbestos-exposed subgroups: no lesion, pleural plaque, benign hydrothorax, asbestosis lung, lung cancer, and malignant mesothelioma.
Then, we studied the responses of two potent sources of inflammatory mediators in the lungs: lung epithelial and alveolar macrophage cells, under a hypoxic condition mimicking atelectasis.
For the determination of weight loss, Mtb load in the lungs, lung pathology, and lung cell viability mice of both sexes were used.
They're just not the best lungs (lung-and-a-half, really), so I quit smoking on Halloween 1988, and I have several slip dreams a week.
Tissue specific, MDA-MB-231-Bone (bone-seeking) and MDA-MB-231-Lung (lung-seeking) cells were the kind gift of Dr. Massague (Memorial Sloan Kettering, New York, NY) as defined previously [ 20, 21].
All patients presented extensive pathological alterations in the lungs; lungs were heavy, diffusely edematous and with variable degrees of hemorrhage.
