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In control experiment, we found that the transplanted SOX9+ progenitors cannot incorporate into non-injured healthy mouse lung or porcine pancreatic elastase-injured mouse lung (data not shown).
By 60 days post infection, no bacilli were found in any evaluated organs other than the lung (data not shown).
We have also observed that intratracheal administration of LTA to mice resulted in substantial neutrophil infiltration into the lung (data not shown).
The atypical CnA band was not observed in other organs including mouse brain (Fig. 1A), liver, heart, kidney and lung (data not shown).
At a histological level, when administered to either 5-wk or 20-wk mice, a difference in the quantitative liver (p = 0.28 0.84) or lung (data not shown) cytopathies was not observed when comparing the effects of either GCase.
Expression was detected in the heart, the retina, the ovary and the testis (Fig. 2E, F, H J), but not in the liver, the brain, the spleen, the pancreas, or the lung (data not shown).
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Although Notch2 and Dll1 mRNAs were not induced by Tg6F in both the small intestine and lungs (data not shown), we observed that both Notch2 and Dll1 proteins were increased by Tg6F in the jejunum but not in lung tissue (Fig. 5).
In others, despite lack of clinical symptoms, virus was present in the lungs (data not shown).
However in our metastasis model, these cells are F4/80- and their recruitment is not significantly different in metastasis bearing lungs compared with normal lungs (data not shown).
Fluorescent B16F1 cells were visible within lung sections (Figure 4A - green), with no difference in total tumor cell numbers between Cd34+/+ and Cd34−/− lungs (data not shown).
These discrete foci intensely labeled with Cby or acetylated-α-tubulin were no longer noticeable in adult peripheral lungs (data not shown).
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