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At the lumen face, 13.7 Å separate chlorophyll 3010 from chlorophyll 1114.
The vessel endothelium was usually a single layer of cells, each with a large nucleus extruding toward the lumen face.
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For en face staining, the tissue-engineered vessels were cut longitudinally and mounted with the lumen facing up before incubation with Alexa Fluor 488 phalloidin and/or 4′-6-Diamidino-2-phenylindole (DAPI).
The aorta was dissected free of external fat and connective tissue, cut longitudinally, and mounted en face, lumen side up, on polylysine and 3-aminopropyltriethoxysilane (APES -coated slides (Bolster, WuhAPES -coated
These include GPR109A, a receptor in the lumen-facing apical membrane of colonic epithelial cells that binds butyrate with low affinity.
For the ex-vivo colon tissue, the lumen side was placed facing down (against the cover glass), since the light illuminates from below the sample as seen in the inset of Fig. 2.
Figure 9 shows the fresh artery stained with H&E and MT and all the images are facing the lumen side.
During the experiment, each tissue specimen was placed on a quartz slide with the internal lumen directly facing the microscope objective.
First three required data sets to define design domains are expressed as contour maps in terms of the forward current and the junction temperature (If and Tj): (1) face lumen and cost requirement as lumen/LED; (2) power consumption and energy requirement as luminaire efficacy (LE); and (3) reliability requirement as L70 lifetime.
Lumens typeface.
Protease protection assays suggest that AtCLO3 caleosin is an integral membrane protein arranged in a type-I orientation [ 56], with the N-terminal domain facing the lumen and the C-terminal domain facing the cytosolic side [ 22].
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