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However, there are other reasons why the contractile vacuole lumen does not acidify despite the operation of the proton V-ATPase.
The contrast opacification of the false lumen does not reach the threshold required to trigger the scan as seen on on bolus tracker HU-time graph.
In contrast, in the case of normal capillaries, even when there is a decrease in blood flow the vascular lumen does not collapse, and the normal morphology is maintained.
In the example displayed for HPAFII cells the lumen does not appear to be spherical at all, and while the long diameter is indeed comparable to the MDCK example, the transversal cross section seems to be under 10μm, according to the scale bar.
Similar(56)
The administration of O2 into the jejunal lumen did not produce any significant difference in any of the respiratory or hemodynamic variables measured over the course of the experiment.
However, clinical experience has shown that for example small nitinol wires or stents protruding into the vascular lumen do not cause such problems.
But in this case, tumor cells in apparent contact with the lumen do not show an endothelial phenotype.
Especially noteworthy is the fact that even in the true capillaries with diameters of 15 μm or less, the vascular lumen did not close.
We observed that – in many cases – the inflated lumen did not penetrate the region of the cell where the prophase nucleus was located (Fig. 4A′; n=10).
Inability to aspirate blood from the lumens supports the possibility of erosion or perforation [ 8 ], however, free flow in the lumens does not exclude the diagnosis [ 9 ].
Doubling the lumens doesn't double the brightness.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com