Sentence examples for ltd 1 from inspiring English sources

Each PCR was performed with 2 ng of template DNA in a 20-μL final volume composed of 10 μL 2× PCR Master Mix (Tiangen Biotech [Beijing] Co., Ltd), 1 μL of each primer (final concentration 0.4 μmol/L), and 8 μL of distilled water.

Hippocampus-based learning and memory is likely to be encoded by two forms of hippocampal synaptic plasticity: long-term potentiation (LTP) and long-term depression (LTD) [1] [2].

Homeostatic metaplasticity adjusts the strength of synapses to prolonged changes in postsynaptic neural activity by dynamic modification of the thresholds for inducing LTP or LTD [1], [6].

A large body of evidence has implicated NMDA-type glutamate receptors (NMDARs) in the process of learning and memory, at least in part, due to their contribution to long-term changes in synaptic strength in the forms of long-term potentiation (LTP) and long-term depression (LTD) [1].

The tissue was then incubated with VEGF (C-1), a mouse monoclonal antibody (Mitaka Biotechnology Co., Ltd) (1 50 dilution, Santa Cruz Biotechnology, Santa Cruz, USA) overnight at 4°C, and labeled with Streptavidin-Biotin Complex for detection.

The reaction mixture contained 1 unit of Platinum® Taq DNA polymerase (Invitrogen Life technologies Ltd), 1× PCR buffer, 200 μM each dATP, dCTP, dGTP and dTTP, 2.5 mM MgCl2, 0.2 μM of each specific primer, 1 μl of MAP genomic DNA extract and nuclease free water up to 20 μl.

The reaction mixture consisted of 2 units of Platinum® Taq DNA polymerase (Invitrogen Life technologies Ltd), 1× PCR buffer, 200 μM each dATP, dCTP, dGTP and dTTP, 1.5 mM MgCl2, 0.2 μM of each specific primer, 3 μl of MAP genomic DNA extract and nuclease free water up to 40 μl.

The reaction mixture consisted of 1 unit of Platinum® Taq DNA polymerase (Invitrogen Life technologies Ltd), 1× PCR buffer, 200 μM each dATP, dCTP, dGTP and dTTP, 1.5 mM MgCl2, 0.2 μM of each specific primer, 3 μl of MAP genomic DNA extract and nuclease free water up to 20 μl.

Present address: Center for Physical Sciences and Technology, LT-02300 Vilnius, Lithuania.

The correlation scale, which usually increases in LT 6 00, peaks at LT 12 00 and LT 14 00 and then decreases.

The database includes electron density profiles for different seasonal and solar activity conditions at 4 typical hours: 00 LT, 06 LT, 12 LT and 18 LT.