The maximum biosorption was attained at biosorbent dosage, 0.5 g/0.1 L. Therefore, the optimum biosorbent dosage was taken as 0.5 g/0.1 L for further experiments.
In the first experiment, 8 female chickens from each line were selected from a total of 72 broilers (36 F and 36 L) for further gene expression profile analyses.
We tested a series of resins and selected Amberlite XAD-4 and Optipore L-493 for further studies.
After 12 h of cultivation, the fermentation broth was transferred to another 250 mL flask that containing 2.0 g l-sorbose for further 12 h of cultivation at 29 °C and 180 rpm.
Based on our previous results, to control the relatively low DO level, the strategy of reducing the aeration rate was applied to 50-L bioreactor for further improvement of TL1-1 production.
The males aged between 12 and 18 months were sampled from six 50-L aquaria for further analyses (Table 2).
To translate our results into future clinical trials, we used the pan-NOS inhibitor L-NMMA for further study.
Then these attached EBs on fibronectin-coated plates were cultured in nonserum culture medium: DMEM/F12 (Invitrogen) supplemented with l-ascorbic acid (50 μg/mL) for further differentiation into CMs.
Furthermore, to reveal the feasibility of Langmuir adsorption isotherm, the dimensionless parameter of the equilibrium or adsorption intensity (R L) was used for further analysis of Langmuir equation.
3. Leave only diagonals with N min or more l-tuple hits for further processing.
