The archaeon was grown anaerobically at 65°C on 80%H2/20%CO2/0.1%1% H2S in a 12-L fermenter containing 10 L complete mineral salt medium (Schönheit et al., 1980).
Group A spleen cells (5 × 10/100 L complete RPMI medium) were incubated with 0.1, 0.3, and 1.0 μg Con A/50 μL, respectively, at 37°C in the presence of 5% CO2 for 54 hr.
CIA was induced by immunization with 100 μ g of rat CII or human CII emulsified with 100 μ l complete Freund's adjuvant (CFA; Difco, Detroit, MI, USA).
This paper proposes a fuzzy l-complete approximation technique based on Willems' behavioural systems theory for a class of hybrid system with symbolic inputs and quantized measurements.
Furthermore, we show that the set of external behaviour (i.e., state set) of the fuzzy l-complete approximation based on the notion of 'guardian states' contains or at least equal to the behaviour set generated by traditional l-complete discrete abstraction.
For coculturing HeLa cells and HMVEC-d cells, EndoGRO-LS complete media was always used as the culture medium.
The endothelial cells were collected and suspended in endothelial cell growth media (EndoGRO-LS Complete Media Kit; Millipore, catalog number-SCME001).
and 1% penicillin streptomycin (Mediatech, U.S.A .. Human dermal microvascular endothelial (HMVEC-d) cells were purchased from ATCC and maintained in EndoGRO-LS complete media (Millipore, U.S.A).
In order to quantitatively investigate HeLa cell movement within monoculture or coculture, we cultured patterned HeLa cells when HMVEC-d cells (in EndoGRO-LS complete media) were present or absent in our SSAW device.
HUVECs were seeded in six-well Bio-Coat cell culture plates (BD Bioscience, San Jose, CA, USA) at a concentration of 1 × 10 cells per ml and maintained in EndoGRO-LS Complete Media Kit (Millipore, catalog number-SCME001), whereas PC-3 were seeded in six-well plate and maintained in RPMI media.
Since (mathcal{L}') is complete, then ((mathcal{E}, d)) is a generalized T-orbitally complete metric space.
