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It is not possible to document all possible LS activities or even to reduce the domain to a small number of generic practices represented as workflow diagrams.
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In high doses, it also inhibits the T-L and C-L activities [ 87].
21 report that bortezomib inhibits both proteasome CT-L and C-L activities in myeloma cells.
Importantly, our study showed that MM cells exhibit higher constitutive levels of T-L proteasome activity than either CT-L or C-L activities (Crawford et al, 2006).
Ischemia induced a robust increase in C-L and CT-L activities whereas LMP2 knockdown caused nearly 6-fold decrease in C-L and CT-L activities compared with the control group.
Delanzomib (CEP-18770) is a boronic acid-based PI that inhibits, reversibly, CT-L and C-L activities [ 105].
We previously reported that UK-101 inhibits the CT-L activity, but not caspase-like (C-L) or trypsin-like (T-L) activities of the purified immunoproteasome (Ho et al, 2007).
This effect did not fully explain the tumor growth inhibition, suggesting a broader spectrum of PLP-L activities.
We further measured the proteasome-dependent proteolytic activities (the caspase-like (C-L) and chymotrypsin-like (CT-L) activities) after MCAO.
In melanoma, cathepsin B and L activities are increased relative to normal skin [ 25], and both the cysteine proteases conferred a metastatic phenotype [ 26, 27].
In addition, legumain, cathepsin B and L activities in the cell lysates were measured by their ability to cleave peptide substrates (Fig. 3).
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