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For each time series, the value of the lowest time point was set to 1. (B ) Plot of relative mRNA abundance for MAN1 from whole head extracts of tim GAL4/+ and tim GAL4/UAS MAN1 flies determined by qRT-PCR (n = 6, Student's t test, ***p < 0.001).
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We found that some genes exhibit very high amplitude rhythms, with no detectable signal at low time points (e.g., Npas2; Figure 2A).
The low transmission time point was at the end of a long dry period, which started in May 2003.
A thick blood smear for identification of Plasmodium infections was taken at each of two time points: November 2003, a low transmission time point and February 2004, a high transmission time point.
Genes were ranked based on differential gene expression for each of the time points and the ten highest and ten lowest for each time point were given a score, lowest being the genes most down-regulated and highest being genes most up-regulated.
Similar to the results on randomly chosen genes, almost all methods have the lowest accuracy at time point 1 (0 minute), indicating the common difficulty for predicting gene expression at this initial time point.
Figure 1 demonstrates that compared with the patients aged less than 45 years, the mean PCS scores for the patients who were ≥ 45 years of age were lowest at every time point.
Values from BALF samples of infected animals were always compared to values from the first sample of the healthy controls, because the age difference was lowest at that time point.
Figure 3 demonstrates that compared with the patients having monthly income more than 1000 MYR, the mean MCS scores for those earning ≤ 1000 MYR per month were lowest at every time point.
WBC count remained low and time points of death were comparable with the irradiation control groups.
Because WBC count remains low and time points of death were comparable with the irradiation control groups in dying animals after allogeneic bone marrow transplantation, we deduced that the cause of death was hematopoietic failure.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com