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Therefore the lowest test concentration showing an inhibitory effect was repeated in 3 parallels.
We found that the uncoated nanomaterial NM-105 had a clear effect on earthworm reproduction at the lowest test concentration (50 mg/kg).
The inhibition rate was calculated and plotted versus test concentrations to afford the IC50, whereas the MIC was defined as the lowest test concentration that completely inhibited the growth of the test organisms.
For the third lowest test concentration, 2.6 mg/L, one measured concentration was below the limit of quantification (LOQ) of 0.25 mg/L and was assigned a value of one-half the LOQ (0.5 × LOQ) in calculating the geometric mean concentration.
Even at lowest test concentration about 80% iron was chelated by HX, CH, and AC extracts.
A significant increase was observed even at the lowest test concentration (5 μg/ml).
Similar(50)
No algal effects were observed at the two lowest test concentrations.
Now the bees responded equally well to all three test odorants at any one concentration, with only the lowest test concentrations of odorants resulting in a significantly lower PER than that recorded for the corresponding training mixture (Fig. 4D, E; McNemar test [2×2 Table] with Bonferroni corrected threshold; in all cases p<0.0056).
However, even though no threshold could be discerned for these chemicals, we did not characterize them as having LDE (because it was not clear that the lowest test concentrations were low enough to be equated to levels of exposure that are normally seen in the environment).
Some of these chemicals (5 of the 22) had been tested using human primary cell data from ToxCast and had showed statistically significant activity across a full range of doses against the specified targets (i.e. they were active even at the lowest test concentrations of ~0.01 µM).
Stage 2: Test for activity at lowest tested concentration.
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