This finding suggests that presence of the invariant restriction site recognition sequence at the beginning of each read (i.e., low 5' sequence variation) caused base calling errors in subsequent cycles, probably because proper sequence phasing on the Illumina Genetic Analyzer is dependent on detecting 12 random nucleotides at the beginning of each sequence.
Our findings confirm extremely low sequence variation among strains within the same human ecotype.
Soc should be located in regions of high sequence variation in suppressor strains (green) and regions of low sequence variation in non-suppressor strains (blue), relative to B6.
Furthermore, the low sequence variation in TgROP38 gene among different T. gondii strains implied that TgROP38 may represent a potential vaccine candidate against T. gondii infection [ 23].
The lower polymorphism rate for STS or other markers may be due to the low sequence variation in soybean and its wild ancestor G. soja.
The low sequence variation of Y chromosome loci produced the inferred phylogenetic trees with weak support values on the nodes (Additional file 1).
The low sequence variation in the plant mitochondrial genome has led to a search for alternative universal DNA barcodes for plants, which has proven difficult [ 3].
The low sequence variation (0 1.2%) within Planktothrix mcyC sequences makes it difficult to detect recombination, since for the majority of methods, a minimum sequence variation of 5% is necessary to obtain substantial power [ 26].
Sequence analysis of the COI amplicon (electronic supplementary material, figure S2) established that members of a species usually showed low sequence variation, averaging 0.43percentnt (s.e. = 0.017%) while congeneric species possessed 18-fold higher mean divergences (7.70%, s.e. = 0.033%).
As the plants used are from a cultivated variety, we anticipated that there may be low sequence variation, however, the complete absence of detected variability even in the large introns and 5' untranslated regions was unanticipated, and reflects the inbred nature of the cultivated variety.
