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* Significance relates to td at lowest seeding density (p < 0.05).
The highest calculated Ab amount released by a single MSC (73.7 pg/cell in 48 h) was obtained at the lowest seeding density (10 cells/well).
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For the low seeding density of this study, PGA constructs cultured in the bioreactor did not outperform static cultures when comparing collagen content after 8 weeks.
In the culture at the low seeding density, an increase in confluence degree accompanied a decrease in growth potential (Rp), being Rp = 0.85 and 0.11 at t = 48 and 672 h, respectively.
Low seeding density along with basic fibroblast growth factor (bFGF) supplementation in the growth medium, which is DMEM-KO, resulted in propagation of more than 1x108 cells within a time period of 15 days from a single umbilical cord.
Therefore, to test this material for NP regeneration, bovine NP cells were encapsulated in 1% w/vol HA hydrogels at either a low seeding density (20 × 106 cells ml−1) or a high seeding density (60 × 106 cells ml−1), and constructs were cultured over an 8 week period.
Similar changes were also seen in non-GFP-labeled parental MDA-MB-435 cells freshly obtained from ATCC and in MDA-MB-435-GFP cell cultures set up at low seeding density (data not shown).
As CCE cells at high but not low seeding density lose the renewal properties and differentiate into NPCs, it may be speculated that ROCK inhibition brings about dys-regulation of β-catenin localization induced by cell-cell contact and thereby changes β-catenin-mediated murine ES cell renewal and differentiation [28].
The additional fraction of lipid droplet-forming adipogenic cells induced by Y27632 at low seeding density did not reach the level of adipogenic cells without Y27632 at high seeding density.
Independently of the cell culture vessel used, hPDCs proliferated faster than hBMCs and reached full confluence after 10 days (Fig. 1A) when initially seeded at low seeding density (5000 cells/cm).
The colony formation ability in low seeding density was evaluated 2 weeks post-transfection and the pre-miR-34a transfected cells had around 2 3 times lower colony-forming ability than the scramble precursor transfected cells.
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