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If a patient is in the ICU for over 48 hours, this model gives them roughly a 33% probability of developing new-onset disability – this is the lowest predictor of risk.
Using percent increase MSE to rank variable importance, the top two ranked predictors of black bear activity were day of the year (33.4) and Pacific herring egg mass (26.1), followed by similar, moderate-importance predictors year (14.7), amphipod mass (16.8), and location (16.7) with gray wolf activity as the lowest predictor (3.8).
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Human claudin-low tumor samples were identified using either SigClust [ 22] or the nine-cell line claudin-low predictor.
Prediction of the claudin-low subtype was done using the claudin-low predictor developed in Prat et al [ 27].
Next, claudin-low cell lines were identified using the previously reported 9-cell line claudin-low predictor [ 1].
The nine-cell line claudin-low predictor identified 37 samples (~11%) as claudin-low, including the 28 claudin-low samples previously identified by hierarchical clustering.
Samples identified by SigClust [ 22] or the nine-cell line claudin-low predictor were called claudin-low, regardless of the PAM50 call.
Finally, from the claudin-low predictor, we evaluated the Euclidian distance of each sample to the claudin-low 9-cell line centroid.
Conversely, the nine-cell line claudin-low predictor identified the MaSC and stromal (CD49flow/EpCAM-) subpopulations of Lim et al. [ 24] as claudin-low, and as expected, both subpopulations showed the highest and lowest expression of the up- and downregulated genes that define the nine-cell line claudin-low predictor.
Overall, the nine-cell line claudin-low predictor showed 87.5% sensitivity, 97.0% specificity, and 75.7% and 98.7% positive and negative predictive values, respectively, if the SigClust-defined claudin-low group is considered the gold standard.
However, we acknowledge a potential caveat of the nine-cell line claudin-low predictor, which is that tumors with high stromal content might also be identified as claudin-low.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com