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Freeze new cell lines at the lowest passage possible after clearing quarantine.
The lowest passage WNV available were used for crow virulence studies to avoid incorporating confounding cell-culture related geneticell-culture related
The cells were used at the lowest passage numbers that would provide adequate stocks of cells, they were checked as mycoplasm free, and they were authenticated from source, as required by the UKCCCR ' Guidelines on the use of cell lines in cancer research' (< http://ukcccr.icnet.uk>).uk>
Since it is known that primary normal human cells have a finite lifespan in culture, the choice of cell lines used in this study was dictated by the availability of primary human cells with the lowest passage number attained in culture provided by the Coriell Cell Repositories.
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Micropatterning was as effective as TGFβ1 in up-regulating SM-MHC at low passage; however, micropatterning limited VSMC response to TGFβ1 at both low and high passage.
Low passage hESCs (H9, WiCell) were used.
In all experiments with MEFs, low passage (less than 3 passages) MEFs were used.
Each cell line high passage miRNA array was normalized to its corresponding low passage miRNA array.
Low passage MCF10A cells were maintained as described previously [39], [47].
All experiments in previous figures were performed with low passage LNCaP cells (p<30).
A low passage and culture adapted B31 strain of B. burgdorferi was used for these experiments.
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