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This NF was based on the lowest recommended number of reference genes with the lowest level of variation [ 10].
The lowest level of variation (1.40-fold) was among hexaploids, which are also represented by the lowest number of species (and thus of analysed samples).
> The trnH-psbA and rpoB regions showed the highest and the lowest level of variation and species discrimination of all regions evaluated, respectively (Table 3).
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Gambicin exhibited the lowest levels of variation and FBN9 the least (Table 2).
While the mitochondrial markers are very conservative in anthozoans (Shearer et al. 2002), this low level of variation has previously indicated species-level differences in Palythoa species.
However, even with a low level of variation at this locus, A. means and A. pholeter form a clade, primarily exclusive of A. tridactylum.
Consistent with this data, we detected a low level of variation in the PAS/LOV region of WC-1 that is in contrast to the extensive variation present in the SSRs.
Our hypothesis is that this low level of variation can be attributed to subtle changes in the reproductive mode or the epidemiological structure of the fungus because of the particular mild and dry climatic conditions of this area.
Despite the low level of variation in B. quintana, the 16 diverse isolates were resolved into 7 STs, which represents a favourable degree of discrimination relative to other typing schemes in this species.
The two case studies were designed to demonstrate the utility of DArT markers for studies in which highly variable markers are required for evolutionary interpretation and in which relationships are poorly resolved using DNA sequence data due to a low level of variation.
Because of the low level of variation, the tree obtained is of very low resolution.
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