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And for certain miRNAs, group of stages of high expression level was defined by rejection of null hypothesis between lowest expression value in the group and highest one of the remaining.
Cyan indicates the lowest expression value, and pink indicates the highest expression value.
Expression values were adjusted, with the lowest expression value defined as 0 and other values scaled accordingly.
Fold changes in expression levels were calculated using the MS-Excel macros (Biorad) where the lowest expression value was set to one.
Reference-normalized expression measurements were adjusted by defining the lowest expression value as 0, with subsequent 1-unit increases reflecting an approximate doubling of the RNA [ 3].
The transcript asm, encoding an acid sphingomielinase (ASM), showed the lowest expression value after contact to the phytopathogen (Table 3 and Additional file 2).
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The analyses of caspases expression in different tissues showed that muscle tissue presented the lowest expression values in all genes except for caspase-2 and caspase-3/7-2.
Overall the lowest expression values on chromosome 16 were found in 16q13 (Figure S2) which was therefore identified as significant in the pGSEA analysis.
The lowest expression values are shown in bold.
Tukey HSD post hoc testing revealed that the lowest expression values were observed in patients with metastatic breast cancer, whereas expression levels returned to normal with treatment.
Also, the genes with binding activity, hsp98 and a c2h2 (zinc finger domain protein), showed the lowest expression values after 24 hours of growth in FSCW.
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