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The expression levels were represented by a color scale with green as the lowest expression and red as the highest expression.
To make expression level comparisons between different regions of the brain, we grouped values in quintiles from highest to lowest expression and assigned each quintile a color code that is similar to the code used in heat maps: 81 100 (red), 61 80 (yellow), 41 60 (green), 21 40 (blue), >0 20 (gray), and 0 (white).
Linear quantities were calculated relative to the sample having lowest expression, and data were converted to log2 scale.
In either category (with or without K36me3/K27me3), genes at LOCK regions always have the lowest expression and genes at non-LOCK regions have the highest.
The results showed CNE-2 had the lowest expression and 5-8F had the highest expression of miR-185-3p miR-185-3p miR-185-3pcted for subsequent experiments.
DNA from the four unfavourables with the lowest expression and the four favourables with the highest expression of POU4F2 and two cell lines were tested for unmodified cytosines after bisulphite modification, which would indicate methylation of these loci.
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Overall, our results are consistent with expectations of relaxed constraint on genes with low expression and stronger purifying selection on highly expressed genes46 in all three species.
The most obvious remaining bias from this sort of analysis is that genes with low expression and low variability will not be detected as differentially expressed.
However, their usefulness in the mammalian cell is limited by low expression and rapid degradation.
The mRNA expression below the median was designated as low expression and values above the median as high expression.
The most variable genes were CASP3 (low expression) and LYZ (high expression).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com