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As iron chelators can cause iron depletion of cells [ 62, 63] and thus interfere with cell proliferation after rewarming, their concentration should be kept at the lowest effective concentration; therefore, enhancing/injurious effects of the base solutions should be minimized.
Thimerosal reduced NC cell migration at least as potently and effectively as CH3HgCl (lowest effective concentration in the range of 1 5 nM), whereas inorganic mercury (HgCl2) was about 10-fold less potent.
The lowest effective concentration causing the toxic effect (LOEC) for each leachate type and plant species was estimated.
As trypan blue is a potentially carcinogenic vital dye and its possible long-term side effects are unknown, the lowest effective concentration should be used particularly in childern.
The lowest effective concentration of paraquat was approximately 20 μM and the gene expression was induced at paraquat doses between 20 mM and 20 μM.
MDA-MB-231 Dox-inducible 4E-BP1 cells were transiently transfected with the lowest effective concentration of siRNA derived from titration analysis, which was 25 nM of siRNA target gene or siRNA control using TransIT-SiQUEST (Mirus Bio LLC) according to the manufacturer's instructions.
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Here we demonstrated that it is possible to obtain very good splicing correction with negatively charged lipoplexes in serum-containing medium at ON concentrations as low as 10 nM [30] and data not shown] which is one of the lowest effective concentrations described in the literature [47] [50].
These values were half-maximal activity concentrations (AC50) for all assays except the multiplexed transcription factor assay, for which lowest effective concentrations (LECs) were calculated.
We conducted a pilot study to determine the lowest effective concentrations of pure levobupivacaine and levobupivacaine with fentanyl to provide analgesia after C/S.
Moreover, since the intended application of the PDAs is as dietary supplements in cattle to control EHEC colonization, lowest effective concentrations of the plant molecules are advantageous for economical and palatability reasons.
Conventional methods to delivery therapeutic agents for SCI, for example, administration and intravenous infusion have multiple limitations, including elevated risk of infection, low effective concentration, quick drug degradation and reduced tissue penetration11.
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