Sentence examples for lowest base quality from inspiring English sources

Of these, 6236435 and 5491692 reads contained one or more low quality positions in the left and right end reads (defined as having the lowest base quality scores).

Sequences were discarded if ≥80 consecutive bases had the lowest base quality score (marked '#' in the BWA output), and inserts were discarded if there was polyA in the reference genome within 500bp of the mapped position (<10 % for most tumours).

This pipeline accounts for plasmid similarity, polyA/polyT regions, low base quality and slippage.

Furthermore, the 5′ and 3′ ends of reads often have low base quality scores from the sequencing run.

This pipeline removes sequences with plasmid similarity, polyA/polyT regions, low base quality and slippage signals.

Data for samples with outlier properties such as a low fraction of the genome covered or low base quality scores can be excluded, flagged and/or reprocessed.

An alternative strategy is to correct errors caused by low base quality scores in the read sequences, such as false base calls (mismatches), deletions or insertions.

The reads which had adapter contamination and low base quality (≤ Q20) were removed to retain approximately 66.1 million, 51 million and 35.5 million high quality (HQ) reads in BC, BHS and BDS samples, respectively.

Briefly, SNPs with low base quality, SNPs within 15 base of the end of a contig, SNPs in repetitive sequences, such as insertion sequence elements, and SNPs in homopolymeric tracts were removed.

Depending on the ratio of the two sequences and their sequence similarity, the resulting sequence can be close to random with low overall base quality scores, or may be more similar to one of the original molecules and show low base quality scores/higher error rate for only some positions in the read.

Besides, the low quality reads which the N rate > 0.01 and the low quality base (quality < 5) rate > 0.5 were also filtered out.