Exact(1)
Depending on the DNA dataset, lowering stringency to 24/24 nucleotide matches improved annotation up to 19% (Additional file 2), suggesting that some tags contain nucleotide mismatches to available DNAs, potentially derived from sequencing errors or single-nucleotide polymorphisms (SNPs) in transcript sequences.
Similar(59)
A total of 3,118 predicted ORFs had no BLASTn hits to A. thaliana cDNAs even at lowered stringency levels (e value > 0.001).
A region of the Drosophila cDNA cross-hybridized at lowered stringency with an avian alpha-spectrin cDNA.
A lowered stringency of 1 x 10-30 subsequently found the probable position.
In this study, the function DQer were used to break up all contigs containing over 15% Q-clones after several rounds of end-to-end merging and single-to-end merging with lowered stringency of cutoff values progressively.
This type of lowered stringency could be achieved by involvement of multiple cells or molecules in the signaling events, thus the resultant performance of the automata would be much closer to the naturally observable phenomena.
Although by lowering the stringency of estimated FDR, the sensitivity of SFP detection was increased substantially in CP&RT dataset but simultaneously the observed FDR determined after sequencing also doubled (20%) compared to the estimated value by permutation (Table 3b).
By lowering the stringency criteria to 1.5-fold change in two or more consecutive time-points, we detected some potentially differentially expressed genes in either endoneurium or DRG of diabetic mice.
However by lowering the stringency of estimated FDR to 10% the number of predicted SFPs in CP&RT dataset nearly doubled to 61055 that account 9.6% of the total PM features (Table 1b, Figure 4).
Lowering the stringency of the reaction buffer did not result in any detectable interaction (not shown).
Lowering the stringency will increase the number of hits but may introduce more false positives.
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