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In particular, lowering the temperature to 25°C, changing the hybridization buffer (DIG Easy Hybe, Roche), and decreasing the hybridization volume by applying sample under a raised coverslip (LifterSlip, Erie Scientific) drastically increased signal intensities, lowered background, and reduced false signals (not shown).
The purported advantages of RNA-seq include generation of expression data for individual annotated genes with nearly unlimited dynamic range; ability to comprehensively detect novel transcripts and mRNA variants resulting from alternative promoter usages, splicing, polyadenylation and sequence variation; and lowered background.
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I used a smartphone app to raise and lower background sound levels.
New England has lower background radiation, Colorado is much higher, yet cancer rates in New England are higher than in Colorado – an inverse effect.
The difficulty can be overcome to some degree by achieving lower background contamination, by improving instrument sensitivity, and by finding minerals with abundant parent isotopes.
More importantly, LMBs have a much lower background noise and allow faster reaction rates.
The higher counting efficiency and lower background made it possible to perform the low-level monitoring.
In addition, the detection system has a lower background signal owing to application of superparamagnetic nanoclusters.
The proposed aptasensor has the advantages of higher sensitivity and lower background current.
The obtained dendritic Au/BDD electrode has lower background current, better electrochemical performance and higher capacity to immobilize biomolecules.
Comparison with SPCM and glass beads, SHPCM could provide the more wide dynamic detection linear range and lower background signal.
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