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Ultrasonic vocalizations were recorded using an UltraSoundGate Condenser Microphone (CM16, Avisoft Bioacoustics, Berlin, Germany) lowered 1 cm above the top of the isolation beaker containing the pup.
A scalpel was then mounted in an electronic stereotaxic devise, lowered to the drilled furrow and then further lowered 1 mm, penetrating the neocortex, and gently moved 3 mm caudally.
a.Rating scales from (1) – (7) except for: Very strongly lowered (1) – very strongly heightened (9).
Cells were then counted using a Beckman Coulter viCell, and cryopreserved in freezing media (temperature lowered 1 oC per minute until -80 oC).
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Mouse song lowered 16x for audibility.
M1 array was placed 1 mm rostral and 1.6 mm lateral from bregma, and lowered ∼1 mm from the surface of the brain.
131 Lower Marsh SE1, 020-7261 1353, whatthebutlerwore.co.uk Praise the lord!
It put a neutral rating on United Utilities, 6.5p lower at 666p, but lowered its target from 750p to 735p.
It also lowered its Q3 outlook.
Lower 11 showed up and demanded his berth; Lower 9.
"We substantially lower CO2 emissions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com