Our method of cross-priming amplification (CPA) under isothermal conditions combined with immuno-blotting analysis made the whole detection procedure more sensitivity and lower time-consuming.
However, conventional virus isolation methods generally posses lower sensitivity and are time consuming as compared to other diagnostic methods such as detection of viral genome by RT-PCR, and determination of NS1 antigen and anti-DENV antibody by ELISA.
Overall, traditional purification strategies are considered time consuming with lower yields and the trends are moving towards aqueous two-phase extraction, and purification in ionic liquids and purification based on lipase-lipase interaction [ 10].
Although IDT is considered the preferred diagnostic method among dermatologists, ASIS has several advantages over IDT, such as: no patient risk (no sedation required), less traumatic (no repeated injection required), more convenient (no clipping needed, less time consuming), and lower risk of drugs interfering with test results (concurrent anti-inflammatory/antipruritic therapy) [ 45, 46].
However, they are also expensive and time consuming to produce, resulting in lower adoption rates than might be expected [ 3].
Because ASMedian solver is very time consuming, we only perform tests under lower rearrangement rates and diameters.
The lower the coefficient, the higher the calculation accuracy, but the calculation is more time consuming.
Cooking is time consuming".
Time consuming drive this.
But it is time consuming.
But it was time consuming.
