Sentence examples for lower surface of the insert from inspiring English sources

The cells on the lower surface of the insert were stained with crystal violet, and each trans-well membrane was mounted on a microscope slide for visualization and analysis.

The cells on the lower surface of the insert were stained with hematoxylin for 10 minutes.

The cells remaining on the lower surface of the insert were fixed with 4% paraformaldehyde and stained with crystal violet (Sigma, St . Louis MO, USA).

The cells on the lower surface of the insert were stained with crystal violet and each transwell membrane mounted on a microscopic slide for visualization and analysis.

The cells remaining on the lower surface of the insert were stained using Diff-Quick (Dade Behring, Inc., Newark, DE, USA).

After 24-h incubation, non-migrating cells were removed from the upper chamber with a cotton swab; cells present on the lower surface of the insert were stained with Hoechst33342 (Sigma-Aldrich).

Migration cells on the lower surface of the inserts were counted in five random high-powered fields (HPF, 40 × 10) per membrane, and the average number of migrating cells per HPF was calculated.

The cells were fixed and stained, and the number of migrated cells on the lower surface of the inserts was quantified with Image-Pro Plus software (Media Cybernetics, Rockville, MD, USA).

After co-incubation of neutrophils with the inserts at 37°C for 2 hours, the plates with inserts within the wells were centrifuged for 5 minutes at 50 g and 4°C to dislodge adherent neutrophils on the lower surface of the inserts.

After the cells were incubated for 24 h at 37°C in a humidified incubator with 5% CO2, The invasive cells attached to the lower surface of the membrane insert were fixed in 10% formalin at room temperature for 5 min and stained with 0.05% crystal violet.

The invasive cells on the lower surface of the membrane insert were fixed with 4%% paraformaldehyde for 30 min, permeabilized with 0.2 % Triton X-100 at room temperature for 15 min, and then stained with 0.1 % crystal violet for 5 min.