We observed that lower limits of accuracy CIs were decreased in case of classification by gene sets with less than ten genes.
The accuracy of [99mTc]tilmanocept was also statistically significantly greater than the lower limit of the accuracy of [99mTc]sulfur colloid as used in the Z-0360 study (p = 0.0151).
In the present study method validation was carried out with respect to linearity, lower limit of detection, quantification limit, accuracy, precision, and robustness according to ICH guidelines [21].
Assay validation was carried out according to the FDA Guidance for Validation of Bioanalytical Methods and included determination of specificity, linearity over the concentration range 1.5 1500 ng/mL, lower limit of quantitation (LLO Q, precision, accuracy, matrix effects, extraction recovery and stability.
Validated assay parameters according to FDA guidelines [ 12] were: limit of detection (LOD), lower limit of quantification (LLO Q, linearity, accuracy, precision, recovery and stability of PTX using QC samples prepared in drug-free human control plasma at 6.0, 30.0, and 60.0 pmol/μL.
The method was validated by assessing specificity, selectivity, lower limit of quantification (LLOQ), linearity, accuracy, precision, extraction recovery, and matrix effects following Food and Drug Administration guidelines.
The performance of the analytical method was validated for the following set of parameters: linearity, specificity, accuracy, lower limit of detection (LLOD), lower limit of quantification (LLO Q, inter-day precision and intra-day precision.
As more satellites are added to the constellation, the result converges towards the lower limit of the achievable drag coefficient, and accuracy becomes limited by the quality of the ranging measurements and the probability of the accommodation coefficient.
In addition, method validation results of specificity, linearity, accuracy, precision, lower limit of quantitation (LLO Q, and carry-over demonstrated that this on-line sample pretreatment HPLC/MS system was robust and valid as a practical assay of drugs and metabolites in the biological samples.
This potential technical limitation of qPCR, as well as working at the lower limit of DNA concentrations, could have affected the accuracy of the telomere analysis in the present study.
