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In conclusion, the freezing-thawing procedure significantly affects PS translocation, the intracellular H2O2 level, and DNA integrity in canine semen, which may explain the lower fertilization rate and in vitro fertilization (IVF /intracytoplasmic sperm injection (ICSI) outcome when frozen-thawed spermatozoa are used.
Consistently, Separase mutant mice had a lower fertilization rate (71.94±3.59) than control mice (93.77±2.67) (Figure 1E).
In addition more oocytes were retrieved per cycle with a lower fertilization rate in women with PCOS.
In line with other reports showing that FF-leptin levels are predictive of fertilization rates, lower than normal FF-leptin levels in NOW-PCOS may explain their lower fertilization rate and this may be related to the level of insulin and/or insulin resistance.
For example, Tournaye et al. showed a lower fertilization rate in vitro following PTX treatment, probably due to the nature of the patients selected (moderate male factor infertility versus previous unsuccessful IVF treatment) and/or the protocols of PTX addition (Tournaye et al., 1994).
While examining whether body mass index (BMI) may influence IVF outcome, we could demonstrate that obese patients had a significantly higher prevalence of poor responders as compared to nonobese patients, required longer stimulation, used significantly more gonadotropin ampoules, had lower peak estradiol levels, and showed significantly lower fertilization rate.
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However, a recent preliminary report demonstrated an inverse correlation between serum levels of BPA (maximum of 30 nM) and lower fertilization rates of oocytes derived from women undergoing in vitro fertilization (IVF) (Lamb et al. 2008).
According to these data, one may speculate that abnormalities in the sFas sFasL system are indicative of PCOS pathogenesis, further associating decreased oocyte quality, lower fertilization rates and higher miscarriage rates with PCOS.
They found a trend toward lower fertilization rates in patients with Y microdeletions but it did not reach statistical significance, while the CPR of the microdeletion group was similar to that of controls.
Opøien et al. noted in a large retrospective trial that in comparison to tubal factor controls, patients with ASRM stages I-II disease had lower fertilization rates whereas patients with more severe disease had fewer oocytes retrieved despite requiring higher gonadotropin doses [ 9].
As shown for other mutants, such ookinete formation defects result from lower fertilization rates (caused by infertile male or female gametes; e.g. van Dijk et al., 2001) or abortion of zygote to ookinete transformation (Khan et al., 2005; Reininger et al., 2005; 2009; Tewari et al., 2005; Mair et al., 2006; 2010).
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