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Conservation groups like the Biscayne Bay Waterkeeper argue that this finding alone should have driven the Army Corps to seek a new biological opinion assessment by the National Marine Fisheries Service, rather than following a 2011 opinion that estimated a lower colony count.
Moreover, it can be observed that at the end of experiment, the assays with higher concentrations of extracts (PP 1000 and PL 2000 µg/mL) showed lower colony radius (21.00 and 19.00 mm, respectively) when compared with the control assay (25.00 mm) (p < 0.05).
These data indicate that the substitution of domain III resulted in a replication-competent chimeric replicon, although with moderately decreased (∼7-fold lower) colony forming efficiency compared to neo-RepD.
In primary cultures, α6+/MHCI- cells exhibited lower colony forming efficiency than α6+/MHCI+ cells.
However, when coli-form bacteria (eg, E. coli) are isolated, lower colony counts might also represent significant bacteriuria.
Meanwhile, cells transfected with ΔNp63 siRNA exhibited a significantly lower colony forming efficiency as well as a significantly longer population doubling time than the control cells.
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Although F2 showed lower colony-forming unit in the lungs, avc1Δ did not, indicating this is unlikely to be contributing to the virulence phenotype of avc1Δ.
The colony formation ability in low seeding density was evaluated 2 weeks post-transfection and the pre-miR-34a transfected cells had around 2 3 times lower colony-forming ability than the scramble precursor transfected cells.
H1792 cells showed significantly lower colony-forming activity in soft agar after RASSF7 KD2 siRNA treatment as both the size and the number of colonies was reduced compared with the controls.
We also observed that TPM3 knockdown lowered colony formation and anchorage independent growth.
Possible costs could be lowered colony efficiency if increased aggression interferes with colony routines or even aggression toward nestmates.
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