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The proliferation-driven system requires lower cell death and efflux rates than the influx-driven system.
Therefore, the higher cell viability, i.e., the lower cell death rate of the group treated with both PSi and laser in the present test may be mainly attributed to the lower PSi concentration in the PSi drug solution.
Laforin overexpression reduced the percentage of cells with mutant huntingtin aggregates, which was also associated with lower cell death (Fig. 5C).
WT DJ-1 overexpressing cells exhibited significantly lower cell death following simulated IRI to a similar extent as the insulin-positive control.
Consistently, we observed lower cell death in wild-type cells after 0.35 µg/mL treatments than after 0.25 µg/mL treatments, but not in mutant cells.
Surprisingly, MHH-Med1 cells displayed a stronger caspase activity compared with D283- and D458-MED cells, although lower cell death was observed in these cells.
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We confirm here that caspase 6 levels increase at doses lower than cell death was measured.
It results a change in the equilibrium of pro- to anti-apoptotic molecules that lowers the cell death threshold and strongly favours apoptosis.
A lower PA suggests cell death or decreased cell integrity, whereas a higher PA suggests large quantities of intact cell membranes [40].
In contrast, the RAW264.7-Casp2KD cloneshoweded a significantly lower rate of cell death rate (5 44%) (P<0.05) (Figure 9B).
In addition, we show that the survival of older newborn neurons alone (i.e. those born at the beginning of the enrichment) is higher than in controls, due to the significantly lower levels of cell death.
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