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For both approaches, the experiment has to be performed with two defined culture conditions to be able to discriminate mRNAs that are subject to differential regulation of translational efficiencies from mRNAs that have a constitutive low translation initiation efficiency.
In other words, under conditions of limited eIF4E, weakly translated mRNAs demonstrate weak eIF4F formation and low ribosomal loading resulting in low translation initiation.
The ratio of RPF/RNA was extremely low at 1.75 hpi, suggesting low translation rate.
This equilibrium, referred to as the maximum catenary, collapses for low translation speeds but becomes stable for sufficiently high translation speeds.
However, replacement of the original RBS of argF with sequences with low translation initiation intensity could not achieve the desired l-ornithine production, though cell growth was unaffected.
Results: At the low translation frequency (0.2Hz), with EO, subjects behaved like a noninverted pendulum, whereby the head tended to be stabilized more than the hip, and the legs taking up most of the imposed displacement.
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American publishers have one of the lowest translation rates in the Western world, according to Andrew Grabois, a consultant for Bowker, which tracks the publishing business.
The low translational elongation at the translation beginning step directs the target gene to generate the corresponding protein effectively [ 40].
Since stumpy forms have low overall translation activity [ 29], it is possible that many of the mRNAs that had accumulated in the LW042 and LW032 cell populations were being stored in a translationally inactive form, ready for rapid activation upon uptake by a Tsetse fly.
At their motions hearing last week, the five men generally refused to wear their earphones, so the military installed a speaker nearby to provide a low-volume translation.
What Moller, a friend of a friend, was referring to when he spoke of the burger's origins was the local, centuries-old hot pork sandwich called the rundstück warm – which is a low-German translation of "round piece warm", referring to the sandwich's round bun-like bread.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com