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Residues along the central spine of the solenoid and the CTD have low temperature factors, consistent with their role in stabilizing the core of the molecule, whilst N and C-terminal regions beyond the CTD display higher B-factors for solvent-exposed residues, implying greater flexibility (Fig. 1C).
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TEW-1 has the highest occupancy (1.00) and a correspondingly low temperature factor (35.5 Å; mean B-factor 32 Å), thus suggesting fully occupied and highly ordered TEW-1 localisation.
With the exception of the C-terminal Phe314, these capping residues form a relatively rigid cage, as indicated by their invariant positioning in all EGS crystal structures and low crystallographic temperature factors.
Low crystallographic temperature factors observed in this loop region suggest that the atypically open conformation is stable, and multiple crystal forms ruled out that the WPD loop assumed this conformation as a result of crystal contacts.
Membrane rigidification leads to an increase in the cytosolic concentration of the Ca2+ ion [ 15], which is regarded as a major regulator of low temperature responsive factor.
A proteomic study has shown that a CSP is the low temperature trigger factor in Antarctic Pseudoalteromonas haloplankis and acts as the primary chaperone interacting with newly synthesized polypeptides [ 22].
Cis-elements, such as ABA Responsive Element (ABRE), Dehydration-Responsive Element (DRE), C-repeat Binding Factors (CBFHV), and Low Temperature Responsive Element (LTRE) of Clade-XIV genes' promoters were shown in Figure 7.
At low temperature, the Q factors of the SPP modes are higher than that of the optical mode (dipole mode) due to low metallic absorption loss and relatively low optical radiation loss, however, they seriously deteriorate with increasing temperature and absorption loss [2 4].
The novel low temperature inducible transcription factors may allow the detection of further pathways controlling the acquisition of freezing tolerance.
- The estimated potential yield losses due to abiotic stresses, namely, drought, salinity, high temperature, low temperature and other factors were reported as 17, 20, 40, 15 and 8%, respectively., Rice production necessitates to be doubled to overcome the increasing demands of the population.
Differential expression and complementary function of AOX and UCP is reported in a variety of fruits, vegetables, and plant tissues, and may be regulated by ethylene, low temperature, and other factors [ 27, 31, 33- 357 58, 58] through different catalytic efficiencies under distinct physiological conditions [ 26].
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