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The resulting search produced 12345, 89432 and 272992 predicted kinase substrates for high, medium and low stringency motifs, respectively.
In the chosen low stringency conditions complex patterns were obtained, with a sharp band of ∼700 bp in cases where the vanA gene was present.
Two related genes have been identified by low stringency Southern blot analysis.
We show that the resin can be used under low stringency conditions for the rapid, unambiguous capture of native Hsp90 in complex with a native client.
Low stringency amplifications were performed with an annealing temperature of 52°C.
Blots were washed twice with low stringency buffer at room temperature and twice with high stringency buffer at 45°C.
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Amplification of glycosyl hydrolases was attempted by using a low stringency-degenerate primer PCR strategy, using total soil DNA and bulk DNA pooled from positive colonies as template.
We evaluated the use of low-stringency single specific primer PCR (LSSP-PCR) for genetically typing Leptospira directly from urine samples of cattle with clinical suspicion of leptospirosis.
low-stringency cycle.
The one low-stringency cycle (LSC) of primary PCR allowed SWPOP-P to anneal to the target DNA and extend towards SP-P.
We opted to use this somewhat low-stringency minimum Q-score cutoff to maximize the number of useful sequence tags.
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