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However, the pipeline using local realignment plus BAQ had relatively low strand bias correlation with other processing pipelines.
The SB and Fisher scores indicated quite strong strand bias at these three given genome positions while GATK-SB indicated a low strand bias for those positions.
Palindromes longer than 200 bp cannot be amplified by traditional PCR using DNA polymerases with low strand displacement activity, nor can they be stably maintained in Escherichia coli.
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The epizootic started at the beginning of March 2011 with a low stranding rate, but gradually increased during this month.
As shown in Figure 4D, the triple mutant had an optimum efficiency between pH 7.0 and 7.5 and the lowest strand-joining activity (5% of input ligated after 1 hour).
For low relaxation strand, typical values of f py /f pu is approximately 0.90 (Collins and Mitchell 1991; ASTM A416/A416M), and thus, test variables for initial prestressing forces used in this study, 0.8 and 0.9f py, were determined to be equivalent to 0.72 and 0.81f pu, respectively.
From the position of this pattern, despite the fact that C is a low confidence strand, its position can be easily determined.
Typical jacking stress for low-relaxation strand in the pretensioning bed is 0.75f pu, which is 75% of ultimate tensile strength of strand (Collins and Mitchell 1991; Zia et al. 1979; Kim et al. 2016).
Interestingly, the WIFTIR sequence of MST3 corresponding to a low-probability strand region is conserved in both YSK1/SOK1 (WTFPPTIR) and MST4/MASK (WSFTTVR) (Table 2).
The calculated secondary structure content (Supplemental Table 3 and Supplemental Fig. 5) underlines the predominantly α-helical and low β-strand content typical for catalase peroxidases and Class I peroxidases in general.
In contrast to humans, however, we find a preference for both C and G at the 5'-end of the low-expressed strand (One-sided Binomial test, p = 0.012 and p = 0.0078, respectively).
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