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To test whether the known TatAyCy substrate YwbN was implicated in the growth kinetics observed at low salinity, experiments were performed with a ywbN mutant.
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A model including genetic diversity and environmental stress explained much of variation in MPS during the chronic low salinity experiment (Phase 2) (adjusted R2 = 0.74, p < 0.0001).
Comparison of high- and low-salinity experimental results shows that more noticeable capillary hysteresis toward water-wetness arose in the low-salinity experiment.
Its expression was not altered in the low-salinity experiment.
This gene was strongly up-regulated during the low-salinity experiment.
On the other hand, core length had no effect on the performance of the low salinity flooding experiments.
A high salinity injection followed by low salinity injection experiment was conducted to evaluate the effect of low salinity injection on oil recovery as a tertiary stage.
Low salinity water injection experiments were performed by diluting saline water taken from the formation (Patil et al. 2008; Shaddel and Tabatabae-Nejad 2015), sea water (Shaker Shiran and Skauge 2012; Wickramathilaka et al. 2011; Zahid et al. 2012) or an artificial solution with desired salt concentrations.
To test this idea, all growth experiments at low salinity were repeated in the presence of 10 µM FeCl3 (Figure S3, panel D) or FeSO4 (Figure S3, panel E).
After three weeks exposure to low salinity (Week 6 of the experiment), the average census size for 1x populations in this stressful environment was 57% smaller than those in the high salinity control environment, while the high diversity AMX lines were, on average, 24% smaller in the stressful environment relative to their controls.
UER original water and its optimum water were then used in this project as the high and low salinity waters in the CO2 WAG flooding experiments.
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