For example, 'microarray evidence' (ECO:0000058) may report results from a high quality experiment with several biological replicates, or from a single low quality experiment.
However, it is strongly advised to identify and to remove low RNA-quality experiments, as they can lead to erroneous results.
However, the 28s/18s ratio does not always provide a sufficient basis for distinguishing high quality from low quality RNA for microarray experiments [ 21, 26, 27, 32, 39- 41].
In order to exhibit the potency of our proposed methods, we conduct a series of extensive experiments on low quality versions or subsets of three popular benchmark action recognition datasets: the KTH [26], the UCF Youtube [48], and the HMDB [29].
RNA samples with RIN number lower than 7 or yield less than 3 μg was identified as low quality and excluded from the experiment.
For example, a poorly designed experiment, with low quality data, would not be very 'quantitative', even with 3 or more replicates!
Also, in order to obtain a high quality, homogeneous dataset, the arrayQualityMetrics Bioconductor package [ 95] was ran on each experiment and low quality CEL files were excluded from further analysis, as were CEL files corresponding to samples from ecotypes other than Columbia-0.
We apologize for this low quality western blot and repeated this experiment (now Figure 2B).
It is possible that less food was available in the lake with the lowest invertebrate biomass (Papoose Lake) than we provided in the low quality food treatment of the mesocosm experiment (which still contained 20% animal matter).
Libraries constructed from ≤100 ng DNA were successful using the linker-amplification protocol for 454 [ 15], but Illumina libraries failed or were low-quality for Experiment 1, but not Experiment 2. Two separate protocols were used – both optimized for recovery from column purification steps [ 28], but employed different template:adaptor ratios in ligation [ 29].
