Reads from regions with errors had low quality, low coverage, or were missing.
Nevertheless, stable or even higher trends have been observed in countries where cervical screening is either absent or of low quality and low coverage [ 3, 4].
Low cost and high quality coverage processes of engineering to improve institutional efficiency through greater linkages with local economic and social development is proposed.
The real world challenges in these datasets include low quality reads and uneven coverage.
Regions with low quality or single read coverage were augmented by PCR and Sanger sequencing of total cellular DNA.
Ntshanga, et. al., conducted an evaluation of the DOTS program in crisis districts in KwaZulu-Natal and showed poor implementation of DOT where low coverage, low quality and high caseloads were associated with poorer outcomes [ 43].
The false-positives were either attributable to false-negative calls in the healthy sample, mostly due to low quality alignments or low coverage, or they were caused by false-positive calls in the diseased sample, often due to alignment artifacts caused by nearby InDels.
The resulting contig clearly shows the transition from one BAC to the other across the TA repeat with low quality sequences and low sequence coverage flanking the repeat.
Parameters were adjusted for fastq files with low coverage or low quality values, and are reported in Additional file 2. In case of paired-end sequencing files, only one of two read files was used.
Mapping the Gh13 genome to the reference tomato H1706 genome yielded 14.7× coverage of the H1706 genome, after removing low quality reads and duplicates, with 97.6% coverage of the reference genome.
