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Gallbladder was not clearly visualized Fig. 3 Endoscopic transpapillary bile duct biopsy specimen shows infiltration of poorly differentiated adenocarcinoma cells beyond muscularis mucosa a low power field image, b high power field image.
At low power field (×40), tissue sections were screened and 5 areas with the most intense neovascularization (hot spots) were selected.
LPF: low power field.
Positively staining cells in each low power field were identified by fluorescence microscopy.
The three categories were determined under × 40 (low power field) magnification field.
Less than 2 megakaryocytes per low power field means megakaryocytic hypoplasia [ 9] or decreased megakaryocyte distribution.
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Manual counting at low power fields was used to quantitate tubular structures and percentage of inhibition was expressed using VEGF control wells as 100%.
For comparison, the amount of positive staining was counted under low power fields.
Figure 4 shows photomicrographs of typical lung sections with low power fields (left).
For quantification, the percentage of positive cells was calculated using the formula [X (6 low power fields of positive staining)/Y (total count per 6 fields) × 100].
Ragged red fibers, COX-deficient fibers and whorled fibers were counted in low power fields corresponding to a 100-fold magnification.
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