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Low plating density also enhanced their proliferation.
We speculate that under certain circumstances (e.g. at low plating density) the transition from an intermediate to a quasi-mesenchymal state may take place (Figure 14).
SP cells preferentially survive at very low plating density, because such clonal growth favors the presence of CSCs [ 54].
According to these functional data, we could show the increased expression of proteins associated with contact inhibition in the antisense-transfected LN18 cells at low plating density.
Knockdown of NDN expression in 253J and LUCC6 was confirmed and did not affect the rate of proliferation nor colony formation on plastic at low plating density.
At high plating density, single cells encounter more non-relatives than at low plating density, which is reflected in lower relatedness among spores in single fruiting bodies at high density.
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Here we report the development of poly l-lactic acid) (poly l-lacticer substracid that suPLLAt serum-free growth of primary motor ananofibery neuronsubstrateslathat densupport
When we have varied the plating densities from 250 to 15000 cells/cm2, we have found that the dependence is bell-shaped: at very low plating densities the number of osteoclasts formed are directly proportional to the plated monocyte number, whereas very high monocyte concentration becomes inhibitory for osteoclast formation (Fig. 5E).
In addition, the low plating densities of spores of the high relatedness populations could be more susceptible to drift.
At low plating densities, mERhigh cells clearly showed much higher levels of mER than did mERlow cells (Fig. 5, closed circles and open circles, respectively).
The true clonogenic/stem cells are capable of forming colonies of fibroblast-like cells at very low plating densities, and cell contacts would even inhibit cells growth.
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