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It is essential that the cells be well-maintained and of relatively low passage number.
Low passage, actively growing cells work best for infection (less than 12-13 passages).
Low passage HepG2 cells were transduced with NTCP-expressing lentiviruses and selected with 500 μg/ml G418.
Low passage hESCs (H9, WiCell) were used.
A low passage and culture adapted B31 strain of B. burgdorferi was used for these experiments.
We considered carefully the choice of low passage cell lines vs. intact tumors for these experiments.
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To the south, low-passage was blocked by a mammoth power plant on the Queens shore.
It has been proposed that, while low-passage mesenchymal stem cells (MSCs) can generate ARMS, low-passage myoblasts can form ERMS [34 36].
Comparison of low-passage isolates confirms that Rickettsia was present in the culture (Fig. 5).
Mice were subcutaneously injected in the left flank with low-passage human LM1 and Karpas422 DLBCL cells.
All experiments were performed with virulent, low-passage strains obtained by passage through golden Syrian hamsters as described earlier [11].
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