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This relative low number of pathways producing heterologous compounds is related to the fact that heterologous maps usually show a degree of hierarchy higher than the one observed in native metabolic networks such as central metabolism (a comparison is provided in supplementary Additional file 1, Figure S1).
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Furthermore, a low number of tested pathways contributed to lower statistical complexity as well, limiting the number of applied tests.
Interestingly, a relatively low number of 564.41 pathways on the average were found for the central carbon metabolite pyruvate.
By focusing on the most deviant values of any pathway one can identify the cell types that show very high or very low number of active reactions for that pathway compared to other cell types, and can thereby identify the cell type-specific branches of those pathways.
The reason for the low number of SCNA affected genes and corresponding pathways in the latter case may be the high stringency criteria employed in their analysis [13].
The comparably low number of DGAT homologs indicates that this pathway for TAG generation could in vivo be less adaptive in M. neglectum.
We further hypothesized that the virus tendency to target host pathway hubs effectively mediates the infection while ensuring variety, such that the virus targets numerous distinct pathways with a comparably low number of targeted proteins.
Besides that, the low number of reports about using the B. subtilis MEP pathway to produce terpenoids highlights the need for more research in this area.
Given the low number of significantly differentially regulated genes obtained (42 genes), Ingenuity Pathway analysis was run with a q-value <12%9696 genes).
This procedure is able to address situations where pathways are roughly defined, include genes that are not necessarily co-expressed or when the regulation of the pathway is concentrated in a low number of switch genes.
Thus, the low number of plastid genes of possible non-cyanobacterial origin (< 10%) in mesophilic Plantae makes it unlikely that the pre-endosymbiosis HGT scenario best explains the extensive phylogenetic mosaicism of the plastid AAB pathways (i.e., 66% of non-cyanobacterial enzymes).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com