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However, low negative effects of SU6668 on Shh dependent activation of Gli1 in the case of restored levels of Gli2 observed 96 h after Ulk3 RNAi may be caused by possible instability of SU6668 in cell culture conditions during 48 h of incubation and consequently, insufficient concentration of active SU6668 per Gli2/Ulk3 complex in the cells expressing increased levels of Gli2 protein.
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TB service had the largest negative effects on TB prevalence in Tibet and Xinjiang, with the coefficients −0.291 to −0.240, and the lowest negative effects on TB prevalence in Zhejiang and Fujian, with the coefficients −0.107 to −0.098 (Fig. 4).
Economic level had the largest negative effects on TB prevalence in Zhejiang, Fujian, Guangdong, Guangxi and Hainan, with the coefficients −0.488 to −0.465, and the lowest negative effects on TB prevalence in Xinjiang, with the coefficients −0.371 to −0.293 (Fig. 7).
Geographic factor had the largest negative effects on TB prevalence in Jiangsu, Zhejiang and Fujian, with the coefficients −0.372 to −0.363, and the lowest negative effects on TB prevalence in Tibet and Xinjiang, with the coefficients −0.262 to −0.192 (Fig. 10).
Health level had the largest negative effects on TB prevalence in Zhejiang, Fujian, Jiangxi, Guangdong, Guangxi and Hainan, with the coefficients −0.727 to −0.687, and the lowest negative effects on TB prevalence in Xinjiang, with the coefficients −0.538 to −0.458 (Fig. 6).
Health investment had the largest negative effects on TB prevalence in Inner Mongolia, Heilongjiang and Xinjiang, with the coefficients −0.393 to −0.379, and the lowest negative effects on TB prevalence in Fujian, Guangdong, Guangxi, Hainan and Yunnan, with the coefficients −0.347 to −0.331 (Fig. 5).
In plant breeding programs, mutagenic treatments with low negative physiological effects and strong genetic effects are desirable.
This is likely because highest temperatures often occur when humidity is low and negative effects of high temperature on pre-adult vector life stages may limit adult abundance.
We also found that low salinity had negative effects on other fitness-related traits including initial growth and shell strength, although mortality was also lowest in low salinity.
Therefore, rural kindergarten teachers' social status and welfare are relatively low, which has negative effects on the job stability of rural kindergarten teachers.
Despite better performance in low and intermediate salinities in terms of e.g. maximum size and survival, our experiments revealed that long-term exposure to low salinity had negative effects on other fitness-related traits in B. improvisus.
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