Under these conditions, and using the 5′-C XG-3′ template (top panel), insertion of dNTPs after 30 min was minimal, but a preference for low levels of mis-insertion of dATP and to a lesser extent, dGTP, were observed.
However, the vaccine-type strains showed a low level of BteA protein expression, suggesting that insertion of IS481a represses P1 promoter activity, and that P2 transcript has a low translational efficiency from the additional nucleotide sequence (79 nucleotides) at its 5' end.
This behavior appears suitable for platforms that produce low levels of spurious insertions and deletions during sequencing, such as the Illumina platforms.
The low level of Tau overexpression from our 68A insertions results in moderate toxicity and provides a baseline to investigate factors that increase toxicity.
This likely also explains the asymmetric H3K9me3 distribution around mdg1 insertions in control cells, as even here, a low level of Pol II occupancy is detected right downstream of the insertion site (e.g., Figure 5B).
This showed that all the insertions reproducibly maintained a low level of DNA methylation.
In this species, the vast majority of nLTR-RT insertions are relatively young, as suggested by their low level of divergence.
The point is that gene's TP can be split up by insertions and deletions into several sections that may have rather low level of Z, but which is greater than 5.0.
Despite a relatively low level of retrotransposition, the Alu Ye lineage has contributed to the architecture of the human genome through insertion mutations, retrotransposition associated genomic deletions, and gene conversion.
I claim a low level of boredom".
