Interestingly, the two CD4+ T-LGL that were slightly different from the other 11 CD4+ T-LGLs based on Jβ1.5 usage did show low level clustering with two CD8+ T-LGLs, as evidenced from the common Jβ1.5 and Vβ8/Vβ13 gene usage, and the CDR3 sequence logo.
Although BPD measure is optimum for forming the lowest level cluster (i.e., species level), it could not establish the phylogenetic relationship of the isolates examined.
While this approach is a suitable complement to low-level clustering, the resulting display may not resemble the features derived from the reduced dimensionality due to distortions incurred from the incremental movement process, especially in regions of the reduced space that are very dense with samples.
We note that although TCR clusters are not readily visible at the low antigen densities in these experiments, low level TCR clustering has been reported in the resting state by other methods (Schamel and Alarcon, 2013).
The intraclass correlations were ≤0.1, indicating a low level of clustering within school classes, except for total physical activity for which the coefficient was larger (0.09-0.24).
The Intraclass Correlation Coefficient (ICC) was 0.24, indicating a low level of clustering in which the within-farm variation of the C. perfringens count accounted for almost a quarter of the total variation of the count.
The low level of clustering of specialists' attitudes indicates that the attitudes towards part-time work are mostly individually based and suggests only a small effect of social influences and shared circumstances within a partnership beyond individual characteristics.
A low level of clustering (i.e., spatial autocorrelation) was seen in the examination of residuals: values of Moran's I were in the range 0.06 to 0.12 for BS and 0.03 to 0.09 for SO2, with p < 0.05 in all cases.
The Intraclass Correlation Coefficient (ICC) that compared the within-farm and between-farm variance was 0.24 and 0.25, respectively for linear regression and logistic regression, indicating a low level of clustering in which the within-farm variation of C. perfringens count and the presence of cpb2 accounted for almost a quarter of the total variation.
This was due to the low level of clustering effect found in the trials (DAD Intra Cluster Correlation, ICC = 0 for all outcomes, Diabetes ICC = 0 to 0.01 for all outcomes except OPTION ICC = 0.3, and Statin Choice ICC = 0.04 to 0.09 for all outcomes except OPTION ICC = 0.2.
