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We and others have previously shown that [99mTc(CO)3]+ can bind non-specifically with a low labelling efficiency (approximately 10%) to proteins in the absence of a His-tag [12, 2].
Direct labelling of non-His-tag proteins with [99mTc(CO)3]+ has previously resulted in poor stability and low labelling efficiency and specific activity, indicating that other potential amino acid side chains donor groups such as thiol, thioether, carboxylate and amine do not make a significant contribution in the absence of histidines [3 9].
Therefore the very low labelling detected for the 3'H RNA (lanes 2 and 4; less than 1% of the signal registered for lanes 1 and 3) demonstrated the efficiency of the 3'end modification of these RNAs.
We observed a very low labelling index in PBS prostate tissue.
LGR-5 expression in NM was low (Labelling Index (LI): 0.22±0.03 (means±SE)) with positive cells located mainly at the base of the crypts.
In contrast, after hydralazine almost 50% of vessels had no surrounding IdUrd labelling, whereas after nicotinamide there were fewer vessels with low labelling and a higher median score.
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The new contrast agent showed efficient cellular uptake and significant contrast enhancement at very low labeling concentrations (0.5 μM).
Thus, for the above mapping, we can map the CPU ranges [16 23] to the Very Low label, [20 33] to Low and [40 54] and [48 56] to Medium.
The aim of this study was to estimate the performance of active learning algorithm for detecting blueberry damage using hyperspectral transmittance data with the very low labeling cost.
In video clip 14, a low labeling rate was achieved because images were captured in the gradually dimming light of dusk.
These residues, however, are prone to deiodination after in vivo administration [28] and generally result in low label stability and loss of free iodide.
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