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Genes with low fold change differences should certainly be excluded, at least at the stage of initial examination of the results.
From dark green to light green = low fold change downregulation to more down regulation.
Genes are ranked from high to low fold change (differential expression in DLBCL versus remainder of samples).
Genes are ranked from high to low fold change (differential expression in FL versus remainder of samples).
Genes are ranked from high to low fold change (differential expression in reactive versus remainder of samples).
Genes are ranked from high to low fold change (differential expression in cHL versus remainder of samples).
Similar(38)
Targets not confirmed by RT-qPCR (i.e., GSTM2 and RPL5) were significantly confirmed by droplet digital (ddPCR), which may represent a superior method when confirming DEGs with low fold changes.
Relatively low fold changes of upregulation for Gli2 (4 fold), as compared to Ascl1 (1400 fold) or NeuroD (120 fold), are due to high levels of Gli2 expression in undifferentiated cells (Fig. 2F) [73].
Another interesting measure of microarrays performance is their sensitivity in detecting relatively low fold changes.
The problem is exacerbated for transcripts with small differences in expression (low fold changes).
The downregulated genes showed low fold changes ranging from 1.6 to 2.0.
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